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Article

A directed approach for engineering conditional protein stability using biologically silent small molecules.

The Journal of biological chemistry - 24 Aug 2007

Maynard-Smith Lystranne A, Chen Ling-Chun, Banaszynski Laura A, Ooi A G Lisa, Wandless Thomas J

Abstract excerpt

The ability to regulate the function of specific proteins using cell-permeable molecules can be a powerful method for interrogating biological systems. To bring this type of "chemical genetic" control to a wide range of proteins, we recently developed an experimental system in which the stability of a small protein domain expressed in mammalian cells depends on the presence of a high affinity ligand. This...

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Identifiers

PMID: 17603093
DOI: 10.1074/jbc.M703902200
PMCID: PMC3290522

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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