Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Detection of two functional polymorphisms in the promoter region of the IL-18 gene by single-tube allele specific PCR and melting temperature analysis.

Journal of immunological methods - 1 Sept 2005

Boniotto Michele, Segat Ludovica, Milanese Michele, Crovella Sergio

Abstract excerpt

IL-18 is an important cytokine in both innate and acquired immunity. IL-18 promoter polymorphisms have been investigated in several autoimmune and infectious diseases. Here we present two assays for a medium to a high throughput genotyping of C-607A and G-137C IL-18 SNPs. Using these protocols we have been able to rapidly genotype a cohort of 131 healthy Italian individuals. The assays, based on a multiplex...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Alleles
Share to Child

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Hot Temperature

Share to Interleukin-18

Share to Polymerase Chain Reaction

Share to Polymorphism, Genetic

Share to Polymorphism, Single Nucleotide

Share to Promoter Regions, Genetic

Source

Identifiers

PMID: 16111697
DOI: 10.1016/j.jim.2005.06.016

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Detection of two functional polymorphisms in the promoter region of the IL-18 gene by single-tube allele specific PCR and melting temperature analysis.PMID 16111697Read ↗

Rapid detection of functional gene polymorphisms of TLRs and IL-17 using high resolution melting analysis.Semantic proximity Read ↗

Cloning and mutation analysis of the human IL-18 promoter: a possible role of polymorphisms in expression regulation.Semantic proximity Read ↗

Association of single nucleotide polymorphisms in the IL-18 gene with production of IL-18 protein by mononuclear cells from healthy donors.Semantic proximity Read ↗

Interleukin 18 -607 A/C Gene Polymorphism is Associated With Susceptibility to IgA Nephropathy in a Chinese Han Population.Semantic proximity Read ↗

Roles of functional polymorphisms in the interleukin-18 gene promoter in sarcoidosis.Semantic proximity Read ↗

An accurate, high-throughput method for genotyping the -373AnTn polymorphism in the interleukin-6 promoter.Semantic proximity Read ↗

Sequence-Specific Primer Polymerase Chain Reaction Genotyping of the Human TLR9 Promoter Polymorphism rs187084 (-1486T>C).Semantic proximity Read ↗

Targeting both rs12979860 and rs8099917 polymorphisms with a single-tube high-resolution melting assay for IL28b genotyping.Semantic proximity Read ↗

Modified low cost SNP genotyping technique using cycle threshold (Ct) & melting temperature (Tm) values in allele specific real-time PCR.Semantic proximity Read ↗

Reduced transcriptional activity in individuals with IL-18 gene variants detected from functional but not association study.Semantic proximity Read ↗

Single-tube genotyping of MBL-2 polymorphisms using melting temperature analysis.Semantic proximity Read ↗

Interleukin-18 gene promoter polymorphisms and recurrent spontaneous abortion.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.