Article
Single-tube genotyping of MBL-2 polymorphisms using melting temperature analysis.
Clinical and experimental medicine - 1 Jul 2002
Hladnik U, Braida L, Boniotto M, Pirulli D, Gerin F, Amoroso A, Crovella S
Abstract excerpt
Recently several authors correlated MBL-2 gene polymorphisms with different pathologies and there is a growing interest for MBL-2 genotyping in a large number of individuals. We have developed a single-tube, rapid, economic, and fully automated melting temperature analysis screening method, based on ABI 7700 Sequence Detection System technology and SYBR Green I chemistry, for the detection of three polymorphisms...
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