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Article

Accurate multiplex polony sequencing of an evolved bacterial genome.

Science (New York, N.Y.) - 9 Sept 2005

Shendure Jay, Porreca Gregory J, Reppas Nikos B, Lin Xiaoxia, McCutcheon John P, Rosenbaum Abraham M, Wang Michael D, Zhang Kun, Mitra Robi D, Church George M

Abstract excerpt

We describe a DNA sequencing technology in which a commonly available, inexpensive epifluorescence microscope is converted to rapid nonelectrophoretic DNA sequencing automation. We apply this technology to resequence an evolved strain of Escherichia coli at less than one error per million consensus bases. A cell-free, mate-paired library provided single DNA molecules that were amplified in parallel to...

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PMID: 16081699
DOI: 10.1126/science.1117389

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

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