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Low-Cost, High-Throughput Sequencing of DNA Assemblies Using a Highly Multiplexed Nextera Process.

ACS synthetic biology - 17 Jul 2015

Shapland Elaine B, Holmes Victor, Reeves Christopher D, Sorokin Elena, Durot Maxime, Platt Darren, Allen Christopher, Dean Jed, Serber Zach, Newman Jack, Chandran Sunil

Abstract excerpt

In recent years, next-generation sequencing (NGS) technology has greatly reduced the cost of sequencing whole genomes, whereas the cost of sequence verification of plasmids via Sanger sequencing has remained high. Consequently, industrial-scale strain engineers either limit the number of designs...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 25913499
DOI: 10.1021/sb500362n

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026. Prepared record publication-25913499-2026-09-04.2. Manifest publication-graph-cohort-2026-09-04.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 4 Sept 2026.

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