Article
Rescue of the complex temperature-sensitive phenotype of Chinese hamster ovary E36ts20 cells by expression of the human ubiquitin-activating enzyme cDNA.
The Biochemical journal - 15 Dec 1994
Handley-Gearhart P M, Trausch-Azar J S, Ciechanover A, Schwartz A L
Abstract excerpt
The ubiquitin conjugation system is a multi-step pathway in which ubiquitin is activated and conjugated to acceptor proteins, one function of which is to target acceptor proteins for rapid degradation within the cell. The conjugation system is involved in many aspects of cellular functions, including the cell cycle. Several cell-cycle arrest mutant cell lines have been characterized and appear to harbour a mutant...
Topics
- Animals
- CHO Cells
- Cricetinae
- DNA, Complementary
- Gene Expression
- Humans
- Ligases
- Phenotype
- Sensitivity and Specificity
- Temperature
- Ubiquitin-Activating Enzymes