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Efficient expression, purification, and characterization of C-terminally tagged, recombinant human asparagine synthetase.

Archives of biochemistry and biophysics - 1 Aug 2005

Ciustea Mihai, Gutierrez Jemy A, Abbatiello Susan E, Eyler John R, Richards Nigel G J

Abstract excerpt

Several lines of evidence suggest that up-regulation of asparagine synthetase (AS) in human T-cells results in metabolic changes that underpin the appearance of asparaginase-resistant forms of acute lymphoblastic leukemia (ALL). Inhibitors of human AS therefore have potential as agents for treating leukemia and tools for investigating the cellular basis of AS expression and drug-resistance. A critical problem in...

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PMID: 16023613
DOI: 10.1016/j.abb.2005.05.023

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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