Article
Use of Clostridium septicum alpha toxins for isolation of various glycosylphosphatidylinositol-deficient cells.
Journal of microbiology (Seoul, Korea) - 1 Jun 2005
Shin Dong-Jun, Choy Hyon E, Hong Yeongjin
Abstract excerpt
In eukaryotic cells, various proteins are anchored to the plasma membrane through glycosylphosphatidylinositol (GPI). To study the biosynthetic pathways and modifications of GPI, various mutant cells have been isolated from the cells of Chinese hamster ovaries (CHO) supplemented with several exogenous genes involved in GPI biosynthesis using aerolysin, a toxin secreted from gram-negative bacterium Aeromonas...
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