Article
Temperature-sensitive yeast GPI anchoring mutants gpi2 and gpi3 are defective in the synthesis of N-acetylglucosaminyl phosphatidylinositol. Cloning of the GPI2 gene.
The Journal of biological chemistry - 2 Jun 1995
Leidich S D, Kostova Z, Latek R R, Costello L C, Drapp D A, Gray W, Fassler J S, Orlean P
Abstract excerpt
To identify genes required for the synthesis of glycosyl phosphatidylinositol (GPI) membrane anchors in yeast, we devised a way to isolate GPI anchoring mutants in which colonies are screened for defects in [3H]-inositol incorporation into protein. The gpi1 mutant, identified in this way, is temperature sensitive for growth and defective in vitro in the synthesis of GlcNAc-phosphatidylinositol, the first...
Topics
- Amino Acid Sequence
- Base Sequence
- Cell Line
- Cloning, Molecular
- DNA, Fungal
- Glycosylphosphatidylinositols
- Humans
- Molecular Sequence Data
- Mutation
- Phosphatidylinositols