Article
Variable control of Ets-1 DNA binding by multiple phosphates in an unstructured region.
Science (New York, N.Y.) - 1 Jul 2005
Pufall Miles A, Lee Gregory M, Nelson Mary L, Kang Hyun-Seo, Velyvis Algirdas, Kay Lewis E, McIntosh Lawrence P, Graves Barbara J
Abstract excerpt
Cell signaling that culminates in posttranslational modifications directs protein activity. Here we report how multiple Ca2+-dependent phosphorylation sites within the transcription activator Ets-1 act additively to produce graded DNA binding affinity. Nuclear magnetic resonance spectroscopic analyses show that phosphorylation shifts Ets-1 from a dynamic conformation poised to bind DNA to a well-folded inhibited...
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