Article
Mutations altering substrate specificity in OHIO-1, and SHV-1 family beta-lactamase.
The Biochemical journal - 1 Jun 1992
Shlaes D M, Currie-McCumber C
Abstract excerpt
The OHIO-1 beta-lactamase does not normally hydrolyse oxyimino-beta-lactam substrates like cefotaxime, ceftriaxone, ceftazidime or aztreonam. We were able to select spontaneous mutants of an OHIO-1-bearing strain of Escherichia coli using the antibiotic substrates listed above by enrichment metho...
Topics
- Amino Acid Sequence
- Base Sequence
- Catalysis
- Cephalosporins
- Chloromercuribenzoates
- Escherichia coli
- Hydrolysis
- Kinetics
- Microbial Sensitivity Tests
- Molecular Sequence Data
- Mutation
- Ohio
- Plasmids