Article
Systematic mutagenesis of the active site omega loop of TEM-1 beta-lactamase.
Journal of bacteriology - 1 Apr 1996
Petrosino J F, Palzkill T
Abstract excerpt
Beta-Lactamase is a bacterial protein that provides resistance against beta-lactam antibiotics. TEM-1 beta-lactamase is the most prevalent plasmid-mediated beta-lactamase in gram-negative bacteria. Normally, this enzyme has high levels of hydrolytic activity for penicillins, but mutant beta-lacta...
Topics
- Amino Acid Sequence
- Ampicillin
- Ampicillin Resistance
- Base Sequence
- Binding Sites
- Ceftazidime
- Cephalosporins
- DNA, Bacterial
- Escherichia coli
- Microbial Sensitivity Tests
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Penicillins
- Structure-Activity Relationship
- Substrate Specificity
- beta-Lactamases