Article
New system based on site-directed mutagenesis for highly accurate comparison of resistance levels conferred by SHV beta-lactamases.
Antimicrobial agents and chemotherapy - 1 Aug 1995
Nüesch-Inderbinen M T, Hächler H, Kayser F H
Abstract excerpt
We developed a system based on site-directed mutagenesis that allows a precise comparison of SHV enzymes under isogenic conditions. In addition, the influences of two different, naturally occurring promoters were examined for each SHV derivative. The system comprised two separately cloned DNA fra...
Topics
- Amino Acids
- Base Sequence
- Cloning, Molecular
- DNA, Bacterial
- Escherichia coli
- Genes, Bacterial
- Klebsiella
- Microbial Sensitivity Tests
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Oligonucleotide Probes
- Phenotype
- Plasmids
- Promoter Regions, Genetic
- beta-Lactam Resistance
- beta-Lactamases