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Article

Construction and characterization of an OHIO-1 beta-lactamase bearing Met69Ile and Gly238Ser mutations.

Antimicrobial agents and chemotherapy - 1 Sept 1997

Bonomo R A, Knox J R, Rudin S D, Shlaes D M

Abstract excerpt

Amino acid changes that influence activity and resistance to beta-lactams and beta-lactamase inhibitors were explored by constructing the Gly238Ser and Met69Ile-Gly238Ser mutants of the OHIO-1 beta-lactamase, a class A enzyme of the SHV family. The Km values of cefotaxime and ceftazidime for OHIO-1 and Met69Ile beta-lactamases were > or = 500 microM. The Km of cefotaxime for the Gly238Ser beta-lactamase was 26...

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Identifiers

PMID: 9303389
DOI: 10.1128/AAC.41.9.1940
PMCID: PMC164040

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Source receipt: pubmed, retrieved 7 Sept 2026.

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