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Article

[Construction of protease resistant mutein of human CNTF and its expression in Pichia pastoris].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology - 1 May 2004

Zhao Hong-Liang, Xue Chong, Xiong Xiang-Hua, Zhang Wei, Zhu Hou-Chu, Liu Zhi-Min

Abstract excerpt

AX15 is a mutein of naturally occurring human ciliary neurophic factor (hCNTF), with improved biological activity, stability and solubility. AX15 is susceptible to protease degradation when expressed in Pichia pastoris. Amino acid sequencing revealed the degradation was occurred behind position 12 and 13 amino acid residues, which constitute a dibasic site, RR. Based on the substrate specificity of KEX2, a KEX2...

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Source

Identifiers

PMID: 15971612

Genes

CNTF

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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