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[Expression and purification of tissue factor pathway inhibitor in Pichia pastoris].

Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae - 1 Apr 2007

Luo Shi-Ping, Song Li-Ping, Yu Bo, Zhang Hai-Ling, Liu Lan-Xia, Leng Xi-Gang

Abstract excerpt

OBJECTIVE: To generate recombinant human tissue factor pathway inhibitor (TFPI) in Pichia pastoris. METHODS: To improve the expression of TFPI, a silent mutation was generated at the specific site of TFPI cDNA. Both wild-type TFPI cDNA and mutated TFPI cDNA were cloned into the expression vector pPic9. The constructed plasmids were subsequently transformed into Pichia pastoris cells GS115 and KM71, and the...

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Identifiers

PMID: 17536273

Genes

TFPI

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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