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Article

In vitro antibody evolution targeting germline hot spots to increase activity of an anti-CD22 immunotoxin.

The Journal of biological chemistry - 7 Jan 2005

Ho Mitchell, Kreitman Robert J, Onda Masanori, Pastan Ira

Abstract excerpt

Recombinant immunotoxin BL22, containing the Fv portion of an anti-CD22 antibody, produced complete remissions in most patients with drug-resistant hairy cell leukemia but had less activity in leukemias with low CD22 expression. Complementarity-determining region (CDR) mutagenesis is used to increase antibody affinity but can be difficult to perform successfully. We previously showed that antibodies with...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 15491997
DOI: 10.1074/jbc.M409783200

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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