Article
High-Throughput Generation of Bipod (Fab × scFv) Bispecific Antibodies Exploits Differential Chain Expression and Affinity Capture.
Scientific reports - 5 May 2020
Nesspor Thomas C, Kinealy Kyle, Mazzanti Nicholas, Diem Michael D, Boye Kevin, Hoffman Hunter, Springer Christine, Sprenkle Justin, Powers Gordon, Jiang Haiyan, La Porte Sherry L, Ganesan Rajkumar, Singh Sanjaya, Zwolak Adam
Abstract excerpt
Generation of bispecific antibodies (BsAbs) having two unique Fab domains requires heterodimerization of the two heavy chains and pairing of each heavy chain with its cognate light chain. An alternative bispecific scaffold (Bipod) comprising an scFv and a Fab on a heterodimeric Fc eliminates the possibility of light chain mispairing. However, unpredictable levels of chain expression and scFv-induced aggregation...
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