Article
A novel secreted ribonuclease from Bacillus intermedius: gene structure and regulatory control.
Molecular & general genetics : MGG - 1 May 2000
Hahnen E, Znamenskaya L, Koczan D, Leshchinskaya I, Hobom G
Abstract excerpt
A second secreted ribonuclease, designated binase II, has been detected in Bacillus intermedius 7P, and its structural gene was cloned and sequenced. Unlike the well-known binase I, a 109-amino acid guanyl-specific enzyme, the 292-residue binase II is closely related to the B. subtilis nuclease Bsn, in structure and in its enzymatic properties. Binase II is also insensitive to inactivation by barstar, an...
Topics
- Amino Acid Sequence
- Bacillus
- Bacillus subtilis
- Base Sequence
- Cloning, Molecular
- Endoribonucleases
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Gene Expression Regulation, Enzymologic
- Genes, Bacterial
- Genes, Reporter
- Genotype
- Kinetics
- Molecular Sequence Data