Article
Production of ribonucleotides by autolysis of Pichia anomala mutant and some physiological activities.
Canadian journal of microbiology - 1 Jul 2004
Lee Jong-Soo, Hyun Kwang-Wook, Jeong Seung-Chan, Kim Jae-Ho, Choi Young J, Miguez Carlos B
Abstract excerpt
Various mutants of Pichia anomala were isolated by ethyl methanesulfonate (EMS) treatment and UV irradiation through cycloheximide resistance and KCl sensitivity. The selected mutant HA-2 accumulated a higher content of RNA and grew faster than the wild-type strain in yeast extract-malt (YM) broth. Autolysis of the HA-2 mutant at 60 degrees C and pH 7.0 for 6 h was the best condition to obtain maximum yields of...
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