Enzymatic properties of mutant enzymes at Trp49 and Tyr57 of RNase Rh from Rhizopus niveus.

doi:10.1093/oxfordjournals.jbchem.a021221

Back to search

Article

Enzymatic properties of mutant enzymes at Trp49 and Tyr57 of RNase Rh from Rhizopus niveus.

Journal of biochemistry - 1 Jan 1996

Ohgi K, Takeuchi M, Iwama M, Irie M

Abstract excerpt

In order to establish the role of Tyr57 and Trp49 in the enzymatic reaction of RNase Rh, several mutant enzymes at Tyr57 and Trp49 were prepared by protein engineering and their enzymatic properties were investigated. Among the four mutant enzymes at Trp49 (W49F, W49Y, W49A, and W49I), W49F showe...

Topics

Amino Acid Sequence
Base Sequence
Endoribonucleases
Hydrolysis
Kinetics
Molecular Sequence Data
Mutagenesis, Site-Directed
Mutation
Nucleotides
RNA, Fungal
Rhizopus
Structure-Activity Relationship
Substrate Specificity
Tryptophan
Tyrosine

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.