Article
[Construction, expression, purification and characterization of mutant of Aspergillus flavus urate oxidase].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology - 1 Aug 2010
Zhang Jinlong, Ren Jun, Li Bing, Liu Shuling, Hou Lihua, Fu Ling, Li Jianmin, Chen Wei
Abstract excerpt
We converted the TGC codon (307-309 bp) of Aspergillus flavus urate oxidase (UOX) gene to a GCC codon by using fusion PCR techniques to produce a C103A mutant. This gene was cloned into expression vector pET-42a (+) and then transformed into Escherichia coli BL21 (DE3). The mutant protein (UOX-Ala103) was expressed in soluble form at high levels after induction with IPTG The expressed rUOX-Ala103 accounted for...
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