Article
Detection of rpoB mutations associated with rifampin resistance in Mycobacterium tuberculosis using denaturing gradient gel electrophoresis.
Antimicrobial agents and chemotherapy - 1 Jun 2005
McCammon Mark T, Gillette John S, Thomas Derek P, Ramaswamy Srinivas V, Graviss Edward A, Kreiswirth Barry N, Vijg Jan, Quitugua Teresa N
Abstract excerpt
Denaturing gradient gel electrophoresis (DGGE) was used to probe for mutations associated with rifampin (RIF) resistance in the rpoB gene of Mycobacterium tuberculosis. DGGE scans for mutations across large regions of DNA and is comparable to DNA sequencing in detecting DNA alterations. Specific mutations are often recognized by their characteristic denaturation pattern, which serves as a molecular fingerprint....
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