Article
[Detection of rifampin resistant mycobacterium tuberculosis complex using denaturing HPLC].
The Korean journal of laboratory medicine - 1 Apr 2008
Nam Youn Hyoung, Lee Sang Hyun, Ahn Young Chang, Cho Min Ho, Jang Won Cheoul, Park Su Min, Kwon Pil Seung, Kim Jong Wan
Abstract excerpt
BACKGROUND: Tuberculosis (TB) remains an important cause of morbidity and mortality throughout the world. The surge of TB has been accompanied by an increase in multi-drug-resistant tuberculosis (MDR-TB). In this study, we developed a denaturing HPLC (DHPLC) method for detecting rpoB gene mutation as a rifampin resistance based on sequence. METHODS: In this study, we used 99 mycobacterial isolates grown in Ogawa...
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