Article
Detection by denaturing gradient gel electrophoresis of pncA mutations associated with pyrazinamide resistance in Mycobacterium tuberculosis isolates from the United States-Mexico border region.
Antimicrobial agents and chemotherapy - 1 Jun 2005
McCammon Mark T, Gillette John S, Thomas Derek P, Ramaswamy Srinivas V, Rosas Ishmael I, Graviss Edward A, Vijg Jan, Quitugua Teresa N
Abstract excerpt
Denaturing gradient gel electrophoresis (DGGE) was used to probe for mutations associated with pyrazinamide (PZA) resistance in the pncA gene of Mycobacterium tuberculosis. DGGE scans for mutations across large regions of DNA and rivals sequencing in its ability to detect DNA alterations. Specific mutations can often be recognized by their characteristic denaturation pattern, which serves as a molecular...
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