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Article

Transmembrane protein topology mapping by the substituted cysteine accessibility method (SCAM(TM)): application to lipid-specific membrane protein topogenesis.

Methods (San Diego, Calif.) - 1 Jun 2005

Bogdanov Mikhail, Zhang Wei, Xie Jun, Dowhan William

Abstract excerpt

We provide an overview of lipid-dependent polytopic membrane protein topogenesis, with particular emphasis on Escherichia coli strains genetically altered in their lipid composition and strategies for experimentally determining the transmembrane organization of proteins. A variety of reagents and experimental strategies are described including the use of lipid mutants and thiol-specific chemical reagents to study...

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Identifiers

PMID: 15894490
DOI: 10.1016/j.ymeth.2004.11.002
PMCID: PMC4104023

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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