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Article

Genetic selection for a highly functional cysteine-less membrane protein using site saturation mutagenesis.

Analytical biochemistry - 15 Jun 2007

Arendt Cassandra S, Ri Keirei, Yates Phillip A, Ullman Buddy

Abstract excerpt

We describe an efficient method for generating highly functional membrane proteins with variant amino acids at defined positions that couples a modified site saturation strategy with functional genetic selection. We applied this method to the production of a cysteine-less variant of the Crithidia fasciculata inosine-guanosine permease CfNT2 to facilitate biochemical studies using thiol-specific modifying...

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Identifiers

PMID: 17481563
DOI: 10.1016/j.ab.2007.03.030
PMCID: PMC1949046

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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