Article
Conditional mutagenesis of a novel choline kinase demonstrates plasticity of phosphatidylcholine biogenesis and gene expression in Toxoplasma gondii.
The Journal of biological chemistry - 11 May 2012
Sampels Vera, Hartmann Anne, Dietrich Isabelle, Coppens Isabelle, Sheiner Lilach, Striepen Boris, Herrmann Andreas, Lucius Richard, Gupta Nishith
Abstract excerpt
The obligate intracellular and promiscuous protozoan parasite Toxoplasma gondii needs an extensive membrane biogenesis that must be satisfied irrespective of its host-cell milieu. We show that the synthesis of the major lipid in T. gondii, phosphatidylcholine (PtdCho), is initiated by a novel choline kinase (TgCK). Full-length (∼70-kDa) TgCK displayed a low affinity for choline (K(m) ∼0.77 mM) and harbors a...
Topics
- Base Sequence
- Choline Kinase
- Deanol
- Gene Expression Regulation, Enzymologic
- Molecular Sequence Data
- Mutagenesis
- Mutation
- Phosphatidylcholines
- Protein Multimerization
- Protozoan Proteins
- Toxoplasma