Article
SecY variants that interfere with Escherichia coli protein export in the presence of normal secY.
Molecular microbiology - 1 May 1992
Shimoike T, Akiyama Y, Baba T, Taura T, Ito K
Abstract excerpt
As an approach for studying how SecY, an integral membrane protein translocation factor of Escherichia coli, interacts with other protein molecules, we isolated a dominant negative mutation, secY-d1, of the gene carried on a plasmid. The mutant plasmid severely inhibited export of maltose-binding protein and less severely of OmpA, when introduced into sec+ cells. It inhibited growth of secY and secE mutant cells,...
Topics
- Alkaline Phosphatase
- Bacterial Proteins
- Biological Transport
- Cell Division
- Escherichia coli
- Escherichia coli Proteins
- Genes, Bacterial
- Genes, Dominant
- Mutation
- Plasmids
- Recombinant Fusion Proteins