Article
A cytoplasmic domain is important for the formation of a SecY-SecE translocator complex.
Proceedings of the National Academy of Sciences of the United States of America - 10 May 1994
Baba T, Taura T, Shimoike T, Akiyama Y, Yoshihisa T, Ito K
Abstract excerpt
An approach to identifying the interaction site of multicomponent protein assembly has been applied to the membrane-bound SecY-SecE complex, which mediates protein export across the Escherichia coli cytoplasmic membrane. A dominant negative secY allele, secY-d1, inactivates SecY but preserves its ability to interact with SecE. Thus, the mutant protein sequesters SecE in an inactive complex. Second site mutations...
Topics
- Alleles
- Bacterial Proteins
- Base Sequence
- Binding Sites
- Cell Membrane
- Cytoplasm
- Escherichia coli
- Escherichia coli Proteins
- Genes, Bacterial
- Immunoblotting
- Kinetics