Article
Importance of transmembrane segments in Escherichia coli SecY.
Molecular genetics and genomics : MGG - 1 May 2003
Shimokawa N, Mori H, Ito K
Abstract excerpt
To assess the functional importance of the transmembrane regions of SecY, we constructed a series of SecY variants, in which the six central residues of each transmembrane segment were replaced by amino acid residues from either transmembrane segment 3 or 4 of LacY. The SecY function, as assessed by the ability to complement cold-sensitive secYmutants with respect to their growth and translocase defects, was...
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