Article
SecY protein, a membrane-embedded secretion factor of E. coli, is cleaved by the ompT protease in vitro.
Biochemical and biophysical research communications - 16 Mar 1990
Akiyama Y, Ito K
Abstract excerpt
SecY is an integral membrane protein, spanning the cytoplasmic membrane of E. coli probably 10 times and required for efficient translocation of other proteins across the membrane. We report here that this protein can be specifically cleaved at the central region of the polypeptide after cell dis...
Topics
- Bacterial Proteins
- Cell Membrane
- Escherichia coli
- Escherichia coli Proteins
- Genes, Bacterial
- Immunoblotting
- Membrane Proteins
- Mutation
- SEC Translocation Channels
- Serine Endopeptidases
- Substrate Specificity
