Changes in conserved region 3 of Escherichia coli sigma 70 mediate ppGpp-dependent functions in vivo.

doi:10.1006/jmbi.1995.0518

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Article

Changes in conserved region 3 of Escherichia coli sigma 70 mediate ppGpp-dependent functions in vivo.

Journal of molecular biology - 6 Oct 1995

Hernandez V J, Cashel M

Abstract excerpt

In Escherichia coli, deletion of relA and spoT results in an inability to synthesize ppGpp, guanosine-3',5'-bis(pyrophosphate), and a loss in the ability to grow on amino acid-free minimal media. Two spontaneous missense suppressor alleles, rpoD(P504L) and rpoD(S506F), able to confer complete prototrophy without the reappearance of ppGpp, were localized to that portion of rpoD coding for conserved region 3.1 of...

Topics

Alleles
Amino Acid Sequence
Bacterial Proteins
Base Sequence
Cell Division
Conserved Sequence
DNA-Directed RNA Polymerases
Escherichia coli
Gene Deletion
Gene Expression Regulation, Bacterial
Genes, Reporter

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Guanosine Tetraphosphate

Lac Operon

Molecular Sequence Data

Mutation

Promoter Regions, Genetic

Sigma Factor

Suppression, Genetic

Source

Open in PubMed

Identifiers

PMID: 7563072
DOI: 10.1006/jmbi.1995.0518

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7563072-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Changes in conserved region 3 of Escherichia coli sigma 70 mediate ppGpp-dependent functions in vivo.PMID 7563072Read ↗

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