Article
A coiled-coil from the RNA polymerase beta' subunit allosterically induces selective nontemplate strand binding by sigma(70).
Cell - 29 Jun 2001
Young B A, Anthony L C, Gruber T M, Arthur T M, Heyduk E, Lu C Z, Sharp M M, Heyduk T, Burgess R R, Gross C A
Abstract excerpt
For transcription to initiate, RNA polymerase must recognize and melt promoters. Selective binding to the nontemplate strand of the -10 region of the promoter is central to this process. We show that a 48 amino acid (aa) coiled-coil from the beta' subunit (aa 262--309) induces sigma(70) to perform this function almost as efficiently as core RNA polymerase itself. We provide evidence that interaction between the...
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