Article
Specific amino acid sequences required for O6-methylguanine-DNA methyltransferase activity: analyses of three residues at or near the methyl acceptor site.
Carcinogenesis - 1 May 1992
Chueh L L, Nakamura T, Nakatsu Y, Sakumi K, Hayakawa H, Sekiguchi M
Abstract excerpt
O6-Methylguanine-DNA methyltransferase plays an important role in preventing tumor induction. To elucidate the significance of a highly conserved amino acid sequence of methyltransferase protein, amino acid substitutions were introduced by site-directed mutagenesis of cloned cDNA for human methyl...
Topics
- Amino Acid Sequence
- Cysteine
- Drug Resistance
- Escherichia coli
- Histidine
- Humans
- Methylnitronitrosoguanidine
- Methyltransferases
- Molecular Sequence Data
- Mutation
- O(6)-Methylguanine-DNA Methyltransferase
- Phenotype
- Valine