Article
Lactose-induced cell death of beta-galactosidase mutants in Kluyveromyces lactis.
FEMS yeast research - 1 May 2005
Lodi Tiziana, Donnini Claudia
Abstract excerpt
The Kluyveromyces lactis lac4 mutants, lacking the beta-galactosidase gene, cannot assimilate lactose, but grow normally on many other carbon sources. However, when these carbon sources and lactose were simultaneously present in the growth media, the mutants were unable to grow. The effect of lactose was cytotoxic since the addition of lactose to an exponentially-growing culture resulted in 90% loss of viability...
Topics
- Alleles
- Gene Deletion
- Genes, Fungal
- Kluyveromyces
- Lactose
- Membrane Transport Proteins
- Mutation
- Osmotic Pressure
- beta-Galactosidase