Article
KlRHO1 and KlPKC1 are essential for cell integrity signalling in Kluyveromyces lactis.
Microbiology (Reading, England) - 1 Sept 2006
Rodicio Rosaura, Koch Sabrina, Schmitz Hans-Peter, Heinisch Jürgen J
Abstract excerpt
Cell integrity in yeasts is ensured by a rigid cell wall whose synthesis is triggered by a MAP kinase-mediated signal-transduction cascade. Upstream regulatory components of this pathway in Saccharomyces cerevisiae involve a single protein kinase C, which is regulated by interaction with the small GTPase Rho1. Here, two genes were isolated which encode these proteins from Kluyveromyces lactis (KlPKC1 and KlRHO1)....
Topics
- Amino Acid Sequence
- Cell Membrane
- Cell Wall
- DNA, Fungal
- Fungal Proteins
- Genes, Essential
- Genes, Fungal
- Genetic Complementation Test
- Green Fluorescent Proteins
- Kluyveromyces
- Molecular Sequence Data
- Mutation
- Open Reading Frames
- Protein Transport
- Recombinant Fusion Proteins
- Saccharomyces cerevisiae
- Sequence Analysis, DNA
- Sequence Homology, Amino Acid