Article
Brucella abortus deficient in copper/zinc superoxide dismutase is virulent in BALB/c mice.
Microbial pathogenesis - 1 Feb 1992
Latimer E, Simmers J, Sriranganathan N, Roop R M, Schurig G G, Boyle S M
Abstract excerpt
The gene encoding the Cu/Zn superoxide dismutase (SOD) of Brucella abortus strain 2308 was identified in a Brucella genomic library utilizing a combination of Western blotting and native gel electrophoresis. The Cu/Zn SOD gene was inactivated in vitro by ligation of a kanamycin resistance gene into the open reading frame encoding SOD. The plasmid born construct was introduced back into B. abortus by...
Topics
- Animals
- Blotting, Southern
- Blotting, Western
- Brucella abortus
- Brucellosis
- Cloning, Molecular
- Mice
- Mice, Inbred BALB C
- Mutation
- Superoxide Dismutase
- Virulence