Article
Construction of Cu-Zn superoxide dismutase deletion mutants of Brucella abortus: analysis of survival in vitro in epithelial and phagocytic cells and in vivo in mice.
Infection and immunity - 1 Jul 1992
Tatum F M, Detilleux P G, Sacks J M, Halling S M
Abstract excerpt
Cu-Zn superoxide dismutase (SOD) deletion mutants of Brucella abortus S2308, a virulent strain, and S19, a vaccine strain, were generated by gene replacement. A deletion plasmid, pBA delta sodknr, was constructed by excising the Cu-Zn SOD gene (Cu-Zn sod) from a 2.3-kb B. abortus DNA fragment of plasmid pBA20-1527 and inserting a 1.4-kb DNA fragment encoding kanamycin resistance into the Cu-Zn sod excision site....
Topics
- Animals
- Brucella abortus
- Drug Resistance, Microbial
- Epithelium
- HeLa Cells
- Humans
- In Vitro Techniques
- Kanamycin
- Mice
- Mice, Inbred BALB C
- Mutation