Article
The Brucella abortus Cu,Zn superoxide dismutase is required for optimal resistance to oxidative killing by murine macrophages and wild-type virulence in experimentally infected mice.
Infection and immunity - 1 May 2005
Gee Jason M, Valderas Michelle Wright, Kovach Michael E, Grippe Vanessa K, Robertson Gregory T, Ng Wai-Leung, Richardson John M, Winkler Malcolm E, Roop R Martin
Abstract excerpt
Two-dimensional gel electrophoretic analysis of cell lysates from Brucella abortus 2308 and the isogenic hfq mutant Hfq3 revealed that the RNA binding protein Hfq (also known as host factor I or HF-I) is required for the optimal stationary phase production of the periplasmic Cu,Zn superoxide dismutase SodC. An isogenic sodC mutant, designated MEK2, was constructed from B. abortus 2308 by gene replacement, and the...
Topics
- Animals
- Brucella abortus
- Brucellosis
- Cells, Cultured
- Female
- Gene Expression Regulation, Bacterial
- Host Factor 1 Protein
- Humans
- Macrophages, Peritoneal
- Mice
- Mice, Inbred C57BL
- Mutation