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An STR melt curve genotyping assay for forensic analysis employing an intercalating dye probe FRET.

Journal of forensic sciences - 1 Jan 2011

Halpern Micah D, Ballantyne Jack

Abstract excerpt

The most common markers used in forensic genetics are short tandem repeats (STRs), the alleles of which are separated and analyzed by length using capillary electrophoresis (CE). In this work, proof of concept of a unique STR genotyping approach has been demonstrated using asymmetric PCR and a fluorescence resonance energy transfer (FRET)-based hybridization analysis that combines fluorophore-labeled...

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PMID: 20840288
DOI: 10.1111/j.1556-4029.2010.01549.x

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