Thermostability of firefly luciferases affects efficiency of detection by in vivo bioluminescence.

Abstract excerpt

Luciferase from the North American firefly (Photinis pyralis) is a useful reporter gene in vivo, allowing noninvasive imaging of tumor growth, metastasis, gene transfer, drug treatment, and gene expression. Luciferase is heat labile with an in vitro halflife of approximately 3 min at 37 degrees C. We have characterized wild type and six thermostabilized mutant luciferases. In vitro, mutants showed half-lives...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Enzyme Stability
• Share to Genes, Reporter