Article
Click beetle luciferase mutant and near infrared naphthyl-luciferins for improved bioluminescence imaging.
Nature communications - 9 Jan 2018
Hall Mary P, Woodroofe Carolyn C, Wood Monika G, Que Ivo, Van't Root Moniek, Ridwan Yanto, Shi Ce, Kirkland Thomas A, Encell Lance P, Wood Keith V, Löwik Clemens, Mezzanotte Laura
Abstract excerpt
The sensitivity of bioluminescence imaging in animals is primarily dependent on the amount of photons emitted by the luciferase enzyme at wavelengths greater than 620 nm where tissue penetration is high. This area of work has been dominated by firefly luciferase and its substrate, D-luciferin, due to the system's peak emission (~ 600 nm), high signal to noise ratio, and generally favorable biodistribution of...
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