Article
Identification of mutant firefly luciferases that efficiently utilize aminoluciferins.
Chemistry & biology - 23 Dec 2011
Harwood Katryn R, Mofford David M, Reddy Gadarla R, Miller Stephen C
Abstract excerpt
Firefly luciferase-catalyzed light emission from D-luciferin is widely used as a reporter of gene expression and enzymatic activity both in vitro and in vivo. Despite the power of bioluminescence for imaging and drug discovery, light emission from firefly luciferase is fundamentally limited by the physical properties of the D-luciferin substrate. We and others have synthesized aminoluciferin analogs that exhibit...
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