Article
Acid-triggered membrane insertion of Pseudomonas exotoxin A involves an original mechanism based on pH-regulated tryptophan exposure.
The Journal of biological chemistry - 3 Jun 2005
Méré Jocelyn, Morlon-Guyot Juliette, Bonhoure Anne, Chiche Laurent, Beaumelle Bruno
Abstract excerpt
Exposure to low endosomal pH during internalization of Pseudomonas exotoxin A (PE) triggers membrane insertion of its translocation domain. This process is a prerequisite for PE translocation to the cytosol where it inactivates protein synthesis. Although hydrophobic helices enable membrane insertion of related bacterial toxins such as diphtheria toxin, the PE translocation domain is devoid of hydrophobic...
Topics
- Animals
- Annexin A5
- Aspartic Acid
- Biotinylation
- Cell Line
- Cell Membrane
- Cell-Free System
- Cesium
- Chlorides
- Diphtheria Toxin
- Dose-Response Relationship, Drug
- Endosomes
- Escherichia coli
- Exotoxins