Article
The extracellular domains of FasL and Fas are sufficient for the formation of supramolecular FasL-Fas clusters of high stability.
The Journal of cell biology - 28 Mar 2005
Henkler Frank, Behrle Eva, Dennehy Kevin M, Wicovsky Andreas, Peters Nathalie, Warnke Clemens, Pfizenmaier Klaus, Wajant Harald
Abstract excerpt
Using fluorescent variants of Fas and FasL, we show that membrane FasL and Fas form supramolecular clusters that are of flexible shape, but nevertheless stable and persistent. Membrane FasL-induced Fas clusters were formed in caspase-8- or FADD-deficient cells or when a cytoplasmic deletion mutant of Fas was used suggesting that cluster formation is independent of the assembly of the cytoplasmic Fas signaling...
Topics
- Adaptor Proteins, Signal Transducing
- Apoptosis
- Caspase 8
- Caspases
- Cytoplasm
- Extracellular Space
- Fas Ligand Protein
- Fas-Associated Death Domain Protein
- HeLa Cells
- Humans
- Macromolecular Substances
- Membrane Glycoproteins
- Membrane Microdomains