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Article

Ligand-linked stability of mutants of the C-domain of calmodulin.

Biophysical chemistry - 1 Apr 2005

Hobson Kenosha F, Housley Nicole A, Pedigo Susan

Abstract excerpt

There is a necessary energetic linkage between ligand binding and stability in biological molecules. The critical glutamate in Site 4 was mutated to create two mutants of the C-domain of calmodulin yielding E140D and E140Q. These proteins were stably folded in the absence of calcium, but had dramatically impaired binding of calcium. We determined the stability of the mutant proteins in the absence and presence of...

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Identifiers

PMID: 15792860
DOI: 10.1016/j.bpc.2004.11.002

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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