Article
Paramecium calmodulin mutants defective in ion channel regulation can bind calcium and undergo calcium-induced conformational switching.
Biochemistry - 13 Jun 2000
Jaren O R, Harmon S, Chen A F, Shea M A
Abstract excerpt
Calmodulin (CaM) is an essential eukaryotic protein that binds calcium ions cooperatively at four EF-hand binding sites to regulate signal transduction pathways. Interactions between the apo domains of vertebrate CaM reduce the calcium affinities of sites I and II below their intrinsic values, allowing sequential opening of the two hydrophobic clefts in CaM. Viable domain-specific mutants of Parameciumcalmodulin...
Topics
- Amino Acid Sequence
- Animals
- Binding Sites
- Calcium
- Calmodulin
- Circular Dichroism
- Ion Channels
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Paramecium
- Protein Binding
- Protein Conformation
- Protein Structure, Secondary