Article
Preferences for the selection of unique tRNA primers revealed from analysis of HIV-1 replication in peripheral blood mononuclear cells.
Retrovirology - 24 Mar 2005
Moore-Rigdon Kenda L, Kosloff Barry R, Kirkman Richard L, Morrow Casey D
Abstract excerpt
BACKGROUND: All human immunodeficiency virus (HIV-1) uses a host tRNALys,3 as the primer for reverse transcription. The tRNALys,3 is bound to a region on the HIV-1 genome, the primer-binding site (PBS), that is complementary to the 18 terminal nucleotides of tRNALys,3. How HIV-1 selects the tRNA from the intracellular milieu is unresolved. RESULTS: HIV-1 tRNA primer selection has been investigated using viruses...
Topics
- Binding Sites
- DNA Primers
- HIV Reverse Transcriptase
- HIV-1
- Humans
- Leukocytes, Mononuclear
- Mutation
- RNA, Transfer, Amino Acid-Specific
- RNA, Transfer, Lys
- RNA, Viral
- Ribonucleoprotein, U5 Small Nuclear