Article
Switching the in vitro tRNA usage of HIV-1 by simultaneous adaptation of the PBS and PAS.
RNA (New York, N.Y.) - 1 Mar 2002
Beerens Nancy, Berkhout Ben
Abstract excerpt
Reverse transcription of the HIV-1 RNA genome is primed by the cellular tRNA(lys3) molecule that anneals to a complementary sequence in the viral genome, the primer-binding site (PBS). Additional interactions between the tRNA primer and the viral RNA were proposed to play a role in reverse transcription. We recently identified an 8-nt element in the U5 region upstream of the PBS that is critical for initiation...
Topics
- 5' Untranslated Regions
- Base Pairing
- Base Sequence
- Gene Expression Regulation, Viral
- Genome, Viral
- HIV Infections
- HIV-1
- Humans
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- RNA
- RNA, Transfer, Lys
- RNA, Viral
- Templates, Genetic
- Transcription, Genetic
- Transfection
- Virus Replication